Data-independent acquisition (DIA) is a mass spectrometry-based technique used in proteomics to comprehensively analyze complex biological samples. It is an alternative to the traditional data-dependent acquisition (DDA) method. DIA overcomes some limitations of DDA, such as biased sampling and limited reproducibility, making it a valuable approach for large-scale protein profiling and quantification.
In contrast to the data dependent acquisition method, which selectively chooses specific MS spectra for MS/MS detection (top 5 or top 10), SWATH methodology comprehensively examines all MS and MS/MS spectra, enabling the detection of nearly all observable proteins in a given sample. SWATH technology partitions the MS spectra into multiple narrow windows, wherein peptides within each window are systematically and equally analyzed, followed by subsequent MS/MS analysis. The SWATH technique combines the rapid, large-scale nature of shotgun proteomics with a level of precision similar to to MRM, ensuring enhanced accuracy.
1. Protein extraction and quantification, trypsin digestion and peptides clean-up.
2. LC-MS micro-flow protocol (90 min) coupled to Sciex Triple TOF 5600+ (64vw SWATH-MS).
3. Protein Identification and quantitation with neural networks AI based software (FDR<1%).
4. Differential Expression Analysis, based on Limma statistical significance tests.
5. Pathways enrichment analysis.
6. Report generation.
Deliverables:
1. A report that includes information regarding your sample/s, the methodologies employed, and a concise overview of the data obtained.
2. Proteins identification files for qualitative and /or quantitative data (Excel workbook) or tsv, csv files.
3. Heatmaps (*PDF files).
4. Enrichment chart (Excel workbook), term genes results and KEGG pathways (png images).
How to order
If you feel that the standard workflow fits your experiment (which should be the case in more than 90% of the situations) you simply select the number of samples and order directly. If your experiment does not fit within this standard approach, please contact us via email.
The price is per 90 min LC-MS/MS run of each sample.
Please note that this is the end-to-end complete proteomics service. You may also purchase specific subcomponents of this service which are listed separately in this category, in case for example you choose to perform yourself some of the operations inhouse.
Data Independent Acquisition (SWATH-MS) for Proteins Quantification
- Product Code: MS01
- Availability: In Stock
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250.00€
Tags: Data Independent Acquisition (SWATH-MS) for Proteins Quantification